5 UVC lamp germicidal effect inspection
The germicidal effect of the UV lamp can be measured biologically, not only to check the germicidal effect of the UV lamp but also to check whether the intensity of the UV lamp has been reduced or failed during the use of the lamp. (1) Place the nutrient agar plate under the UV lamp (1 m from the center of the lamp vertically) and irradiate it for a certain period of time (calculate the irradiation time based on the dose required by the microorganism being irradiated and the intensity of the UV light source), then place the agar plate in a 37 °C incubator for 24h and observe the bacterial growth. If it is close to the control group (not irradiated plate), it means that the UV lamp has failed. (2) Inoculate the 24h broth culture of the bacteria to be examined with deep agar tubes that have been dissolved and cooled to 50 °C. After mixing, pour each tube into different sterilized dishes separately, and after the agar has solidified, remove the lid of the dishes and place them under the UV lamp for different times (5, 10, 20, 40, 60 min), take out one dish each, cover the lid of the dishes and place them in the incubator at 37 °C. The growth of the bacteria was observed for 24-72h to determine the killing effect of UV light on the bacteria or the resistance of the bacteria to UV light.
The wavelength of ultraviolet light is shorter than visible light and cannot be seen by the naked eye. The wavelength of the strong germicidal power is 250~265 nm, while the wavelength of ozone formation is 840 nm. Therefore, the germicidal power of UV can not be judged by the intensity of the orchid light emitted by the UV lamp and the intensity of the ozone smell alone. If you have the conditions, you should measure the intensity of the lamp regularly, and if not, you should establish a registration system for use, and record the time of use day by day to determine whether the use period has been reached. To determine whether to reach the use period. When the use of time exceeds 1000 h should be replaced. Although the lamp at this time still will light up, the surface also looks very normal, but in fact, The disinfection effect is no longer reliable. The average life span of the violet lamp is generally 3000~4000h.